I’m Johannes, a scientist and open-source enthusiast situated in Boston, MA. Currently, I work as a research specialist in the Grigorieff Lab at UMass Chan Medical School/HHMI.
My research interests are the mechanism of mechanosensation during hearing and hematopoietic differentiation and malignancies. I love developing new methods to understand how these things work. At the moment, I believe that cryo-electron microscopy of native tissues is the best path to solve my reserach questions.
I enjoy creating software and visualizations using python, whatever web-technology is hot right now, blender and C++.
If you want to talk about science, software, or anything inbetween you can contact me by email or slack: jojotux123 at hotmail.com.
Defocus Corrected Large Area Cryo-EM (DeCo-LACE) for Label-Free Detection of Molecules across Entire Cell Sections
Elferich J, Shiroli G, Scadden D, Grigorieff N
eLife 2022 Nov 16PubmedbioRxivCode
Molecular structures and conformations of protocadherin-15 and its complexes on stereocilia elucidated by cryo-electron tomography.
Elferich J, Clark S, Ge J, Goehring A, Matsui A, Gouaux E
eLife 2021 Dec 29Pubmed
Putting the Pieces Together: the Hair Cell Transduction Complex.
Holt JR, Tobin M, Elferich J, Gouaux E, Ballesteros A, Yan Z, Ahmed ZM, Nicolson T
Journal of the Association for Research in Otolaryngology : JARO 2021 DecPubmed
Molecular mechanism of prestin electromotive signal amplification.
Ge J*, Elferich J*, Dehghani-Ghahnaviyeh S, Zhao Z, Meadows M, von Gersdorff H, Tajkhorshid E, Gouaux E
Cell 2021 Sep 2Pubmed
Workflows for In-Situ Structural Biology Using DeCo-LACE and cisTEM
New England Cryo-EM meeting 2023 (Yale) 2023SlidesCode
Cryo-EM imaging of biomolecular complexes in their native environment: From the rare mechanosensory transduction complex to the abundant ribosome
Seminar at OHSU 2022SlidesCode
Imaging molecules in their native environment: Cryo-electron tomography of PCDH15 complexes in mouse stereocilia
Gatan webinar 2022RecordingSlidesCode
High-throughput in-situ cryo-EM of hematopoietic differentiation
SBGrid Consortium talk 2021Recording
Scientifc web visualizations
An OpenSeadragon and Blender based visualization of DeCo-LACE montaged 2DTM data.WebsiteCode
The Contrast Transfer Function
A WebGL based interactive visualization of the contrast transfer function.WebsiteCode