Hi,

I’m Johannes, a scientist and open-source enthusiast situated in Boston, MA. Currently, I work as a research specialist in the Grigorieff Lab at UMass Chan Medical School/HHMI.

My research interests are the mechanism of mechanosensation during hearing and hematopoietic differentiation and malignancies. I love developing new methods to understand how these things work. At the moment, I believe that cryo-electron microscopy of native tissues is the best path to solve my reserach questions.

I enjoy creating software and visualizations using python, whatever web-technology is hot right now, blender and C++.

If you want to talk about science, software, or anything inbetween you can contact me by email or slack: jojotux123 at hotmail.com.

Papers

Paper image

Defocus Corrected Large Area Cryo-EM (DeCo-LACE) for Label-Free Detection of Molecules across Entire Cell Sections

Elferich JShiroli GScadden DGrigorieff N

bioRxiv 2022 Jun 14

bioRxiv
Code
Paper image

Molecular structures and conformations of protocadherin-15 and its complexes on stereocilia elucidated by cryo-electron tomography.

Elferich JClark SGe JGoehring AMatsui AGouaux E

eLife 2021 Dec 29

Pubmed
Paper image

Putting the Pieces Together: the Hair Cell Transduction Complex.

Holt JRTobin MElferich JGouaux EBallesteros AYan ZAhmed ZMNicolson T

Journal of the Association for Research in Otolaryngology : JARO 2021 Dec

Pubmed
Paper image

Molecular mechanism of prestin electromotive signal amplification.

Ge J*Elferich J*Dehghani-Ghahnaviyeh SZhao ZMeadows Mvon Gersdorff HTajkhorshid EGouaux E

Cell 2021 Sep 2

Pubmed

Talks

Imaging molecules in their native environment: Cryo-electron tomography of PCDH15 complexes in mouse stereocilia

Gatan webinar 2022

Recording
Slides
Code

High-throughput in-situ cryo-EM of hematopoietic differentiation

SBGrid Consortium talk 2021

Recording

Using computational and experimental methods to understand protein function: Application to proprotein convertases and other disease-related proteins

PhD defense 2015

Slides
Code

Structures

Software

Scientifc web visualizations

Visualization image

The Contrast Transfer Function

A WebGL based interactive visualization of the contrast transfer function.

Website
Code
Visualization image

Grid calculator

A simple tool to calculate how many protein complexes are nescessary to see them on a micrograph.

Website
Code

Random artwork

Focus
Magnification