Hi, I'm Johannes

A scientist and open-source enthusiast situated in Boston, MA. Currently, I work as a research specialist in the Grigorieff Lab at UMass Chan Medical School/HHMI.

My research interests are the mechanism of mechanosensation during hearing and hematopoietic differentiation and malignancies. I love developing new methods to understand how these things work. At the moment, I believe that cryo-electron microscopy of native tissues is the best path to solve my research questions.

I enjoy creating software and visualizations using Python, whatever web-technology is hot right now, Blender, and C++.

If you want to talk about science, software, or anything in between you can contact me by email or Slack: jojotux123 at hotmail.com.

Papers

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In-Situ High-Resolution Cryo-EM Reconstructions from CEMOVIS

Elferich J, Kaminek M, Kong L, Odriozola A, Kukulski W, Zuber B, Grigorieff N

bioRxiv 2025 Mar 31

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Dynamic cytoplasmic fluidity during morphogenesis in a human fungal pathogen

Serrano A, Puerner C, Plumb E, Chevalier L, Elferich J, Sinn LR, Grigorieff N, Ralser M, Delarue M, Bassilana M, Arkowitz RA

bioRxiv 2024 Nov 17

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Cryo-electron tomographic investigation of native hippocampal glutamatergic synapses

Matsui A, Spangler C, Elferich J, Shiozaki M, Jean N, Zhao X, Qin M, Zhong H, Yu , Gouaux E

eLife 2024 Nov 4

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CTFFIND5 provides improved insight into quality, tilt and thickness of TEM samples

Elferich J, Kong L, Zottig X, Grigorieff N

bioRxiv 2024 Feb 28

Talks

In-Situ High-Resolution Cryo-EM Reconstructions from CEMOVIS

11th CCP-EM Spring Symposium 2025 (Nottingham) 2025

Structure Determination in Cell Slices using 2D Template Matching

75th ACA Annual Meeting 2025 (Chicago) 2025

Workflows for In-Situ Structural Biology Using DeCo-LACE and cisTEM

New England Cryo-EM meeting 2023 (Yale) 2023

Cryo-EM imaging of biomolecular complexes in their native environment: From the rare mechanosensory transduction complex to the abundant ribosome

Seminar at OHSU 2022

Structures

Software

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cisTEM

An OpenSeadragon and Blender based visualization of DeCo-LACE montaged 2DTM data.

Scientific web visualizations

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DeCo-LACE viewer

An OpenSeadragon and Blender based visualization of DeCo-LACE montaged 2DTM data.

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The Contrast Transfer Function

A WebGL based interactive visualization of the contrast transfer function.

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Grid calculator

A simple tool to calculate how many protein complexes are nescessary to see them on a micrograph.

Random artwork

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Focus

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Magnification

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Astig X

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Astig Y

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