Hi,

I’m Johannes, a scientist and open-source enthusiast situated in Boston, MA. Currently, I work as a research specialist in the Grigorieff Lab at UMass Chan Medical School/HHMI.

My research interests are the mechanism of mechanosensation during hearing and hematopoietic differentiation and malignancies. I love developing new methods to understand how these things work. At the moment, I believe that cryo-electron microscopy of native tissues is the best path to solve my reserach questions.

I enjoy creating software and visualizations using python, whatever web-technology is hot right now, blender and C++.

If you want to talk about science, software, or anything inbetween you can contact me by email or slack: jojotux123 at hotmail.com.

Papers

Paper image

Cryo-electron tomographic investigation of native hippocampal glutamatergic synapses

Matsui ASpangler CElferich JShiozaki MJean NZhao XQin MZhong HYu Gouaux E

eLife 2024 Nov 4

Pubmed
bioRxiv
Paper image

CTFFIND5 provides improved insight into quality, tilt and thickness of TEM samples

Elferich JKong LZottig XGrigorieff N

bioRxiv 2024 Feb 28

bioRxiv
Code
Code
Paper image

Single molecule studies of the native hair cell mechanosensory transduction complex

Clark SMitra JElferich JGoehring AGe JHa TGouaux E

bioRxiv 2023 Dec 12

bioRxiv
Paper image

Defocus Corrected Large Area Cryo-EM (DeCo-LACE) for Label-Free Detection of Molecules across Entire Cell Sections

Elferich JShiroli GScadden DGrigorieff N

eLife 2022 Nov 16

Pubmed
bioRxiv
Code

Talks

Workflows for In-Situ Structural Biology Using DeCo-LACE and cisTEM

New England Cryo-EM meeting 2023 (Yale) 2023

Slides
Code

Cryo-EM imaging of biomolecular complexes in their native environment: From the rare mechanosensory transduction complex to the abundant ribosome

Seminar at OHSU 2022

Slides
Code

Imaging molecules in their native environment: Cryo-electron tomography of PCDH15 complexes in mouse stereocilia

Gatan webinar 2022

Recording
Slides
Code

High-throughput in-situ cryo-EM of hematopoietic differentiation

SBGrid Consortium talk 2021

Recording

Structures

Software

Scientifc web visualizations

Visualization image

DeCo-LACE viewer

An OpenSeadragon and Blender based visualization of DeCo-LACE montaged 2DTM data.

Website
Code
Visualization image

The Contrast Transfer Function

A WebGL based interactive visualization of the contrast transfer function.

Website
Code
Visualization image

Grid calculator

A simple tool to calculate how many protein complexes are nescessary to see them on a micrograph.

Website
Code

Random artwork

Focus
Magnification
Astig X
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